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By STEVE TURNHAM
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Evan Vucci/AP Photo
WATCH Trump to Gold Star Father: 'I've Made a Lot of Sacrifices'
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In his first response to a searing charge at the Democratic National Convention from Khizr Khan, the father of a Muslim soldier killed in Iraq, that he "sacrificed nothing" for his country, DRESSES Kneelength dresses CristinaeEffe Browse Sale Online Explore For Sale Real For Sale Sale Shop For pXtz9ZNCKX
claimed that he sacrificed by employing "thousands and thousands of people." Also, he suggested that Khan's wife, Ghazala Khan, didn't speak at the convention because she was forbidden to as a Muslim and questioned whether Khan's words were his own.

"Who wrote that? Did Hillary's scriptwriters write it?" Trump said in an interview with ABC News' Discount Nicekicks Buy Cheap Manchester Great Sale COATS amp; JACKETS Jackets Peak Performance CiyJAM
. "I think I've made a lot of sacrifices. I work very, very hard."

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on Thursday, Gold Star father Khizr Khan, with his wife by his side, recounted to the crowd how their son, U.S. Army Capt. Humayun Khan, was killed in 2004 by a car bomb in .

Khizr Khan chastised Trump for seeking to bar foreign Muslims from entering the country, saying their son would not have been able to serve under a Trump presidency.

"Go look at the graves of brave patriots who died defending the United States of America," Khizr Khan said, addressing Trump. "You will see all faiths, genders and ethnicities. You have sacrificed nothing and no one."

Trump appeared to try to brush the speech aside, saying that Khizr Khan "was, you know, very emotional and probably looked like a nice guy to me."

Trump also said, "If you look at his wife, she was standing there. She had nothing to say. She probably — maybe she wasn't allowed to have anything to say. You tell me."

This appears to be Trump tipping his hat to some far-right-wing and nationalist Twitter users who have suggested that Ghazala Khan was silent during her husband's speech because they are Muslim and he prohibits her from speaking.

In an interview with ABC today, Ghazala Khan said she did not speak because she was "in pain."

"Please. I am very upset when I heard when he said that I didn't say anything. I was in pain. If you were in pain, you fight or you don't say anything. I'm not a fighter. I can't fight. So the best thing I do was quiet," she said.

Khizr Khan said he asked his wife of 42 years to speak but she declined, knowing she would be too emotional.

"I invited her, 'Would you like to say something on the stage?' when the invitation came, and she said, 'You know how it is with me, how upset I get,'" he said.

Pressed by Stephanopoulos to identify the sacrifices he made for his country, Trump said, "I think I've made a lot of sacrifices. I work very, very hard. I've created thousands and thousands of jobs, tens of thousands of jobs, built great structures. I've had tremendous success. I think I've done a lot."

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memorial in Manhattan and raising "millions of dollars" for vets.

Paul Rieckoff, the founder and CEO of Iraq and Afghanistan Veterans of America, a nonpartisan group with close to 200,000 members, said, "For anyone to compare their 'sacrifice' to a Gold Star family member is insulting, foolish and ignorant. Especially someone who has never served himself and has no children serving. Our country has been at war for a decade and a half, and the truth is most Americans have sacrificed nothing. Most of them are smart and grounded enough to admit it."

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Figure 2. Increased susceptibility of EDA mice to collagen-epinephrine induced pulmonary thromboembolism. Data represent percentage (%) of mortality after the injection of a mixture of collagen and epinephrine through the tail vein. The mortality was twice as high in EDA compared with EDA or EDA mice. n=39 to 42 mice per genotype.

Because the EDA +/+ mice were more sensitive in the pulmonary thromboembolism model, we hypothesized that EDA + FN might be prothrombotic, playing a special role in promoting thrombus growth. We performed in vitro flow chamber studies with whole blood from EDA WT/WT , EDA +/+ , and EDA −/− mice at venous and arterial shear rates in a parallel plate chamber at 37°C over glass coverslips coated with fibrillar collagen type I ( Cupro Skirt Love Potion Cupro S by VIDA VIDA Shopping Online Cheap Online Store Sale P7k7j
). To determine the size of thrombi, the surface area covered by the mepacrine-labeled fluorescent platelets was quantified. The mean ±95% confidence intervals of 6 different optical fields in 3 separate experiments is plotted in Black and Brown Embroidered Lounge Pants Dolce amp; Gabbana Really Cheap Price nIXGa54UQb
B. We found that EDA +/+ blood formed significantly bigger thrombi covering a larger area than EDA −/− or EDA WT/WT samples when perfused over collagen for 1 minute at shear rate of 1500 s −1 (mean±SEM, 15.5±2.4%, 30.5±5.2%, and 18.7±2.7% for EDA WT/WT , EDA +/+ , and EDA −/− , respectively; ANOVA Test, P ≤0.0001, Bonferroni correction EDA WT/WT versus EDA +/+ , P ≤0.001, EDA +/+ versus EDA −/− , P ≤0.01) suggesting a role of EDA + FN in augmenting thrombus growth. However, at venous shear rate of 250 s −1 the percentage surface area covered were similar among EDA WT/WT , EDA +/+ , and EDA −/− mice (mean±SEM, 7.0±0.9%, 9.2±2.6%, and 10.1±2.2% for EDA WT/WT , EDA +/+ , and EDA −/− , respectively; Leather Slimfold Wallet Flight by VIDA VIDA Shop For Sale Clearance 2018 Newest Sale Best Wholesale Cheap Get Authentic Sknv2xpCW
). These studies suggest that the EDA + FN plays a prominent role only at arterial shear rates.

Figure 3. EDA mice form larger thrombi at arterial shear rate in a flow chamber. Whole blood was perfused in a parallel plate chamber coated with type I collagen. A, Representative single-frame images at venous and arterial shear rates. B, Quantification of the surface area covered by platelets after perfusion.

After finding that the EDA + FN promotes thrombus growth in vitro we asked whether the presence of EDA + FN in platelets and plasma would also accelerate thrombosis in vivo. We first evaluated whether EDA containing FN could enhance platelet adhesion at an early time point after ferric chloride-induced endothelial injury. The number of single platelets adhering within 2 to 3 minutes after injury was not significantly different in EDA +/+ mice when compared with EDA WT/WT or EDA −/− mice (EDA WT/WT =328±54, EDA +/+ =299±33, EDA −/− =317±31) suggesting that the EDA + FN does not promote early platelet interaction with the subendothelium.

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